For the samples sintered from the nanometric micrometric pow

For the samples sintered from the 40% nanometric – 60% micrometric powders, with both compaction procedures, the elastic limit is within the 500–530MPa range. The direct-SPS sample presents a sharper strain hardening, especially in the beginning of the plastic deformation, for a maximum stress of 730MPa and a strain to failure of 17%. For the CIP-SPS sample, these values are 680MPa and 34%, respectively.
Acknowledgements The authors are grateful for the support provided by the French ANR research funding through the MIMIC project (ANR-09-BLANC-0010).
Data Rheological properties on 15 samples of laponite-CMC mixes are determined varying mass concentration of the components (laponite and carboxymethylcellulose) [1,2]. Rheograms are established and a viscoplastic model (Herschel–Bulkley) is fitted. Model parameters are expressed as a function of time or components concentration.
Experimental design, materials and methods
Data The fold changes in gene and protein expression as a result of the scratch wound and/or the addition of lysozyme is presented in Table 1. Fig. 1 shows simultaneous changes in gene and protein expression in ON-01910 that survive wounding in the presence or absence of lysozyme.
Experimental design, materials and methods Lysozyme, found in circulating plasma EVs [1], is an antimicrobial agent [2] thought to contribute to the biological responses to injury within the intestinal epithelium [3-4]. Healthy colonic epithelial cells were cultured in the lab and subjected to a scratch assay to examine the effects of lysozyme on wound healing [1] . Resulting transcriptomic (770 genes) and proteomic (30 proteins) changes in the cultures were simultaneously measured using a digital counting assay.
Acknowledgements We acknowledge the funding from the US Department of Health and Human Services, National Institutes of Health, National Institute of Nursing Research, Division of Intramural Research (1ZIANR000018; PI, WAH; Intramural Training Awards to SKA, PVJ, NDK, NHF, LBS, PAS, CMB, KRW). No other funding or benefits from industry or elsewhere were received to conduct this study. The opinions expressed herein and the interpretation and reporting of these data are the responsibility of the author(s) and should not be seen as an official recommendation, interpretation, or policy of the National Institutes of Health or the United States Government.
Data In this study we performed a genome-wide survey of DNA methylation in MDA-MB-231 triple-negative breast cancer cells exposed to resveratrol [1,3]. To determine the methylated and unmethylated DNA regions in the promoters of genes we used Array-PRIMES method (aPRIMES) [[4], Fig. 1]. The extensive annotation of peaks differentially enriched for DNA methylation and peak comparisons between the MDA-MB-231 breast cancer cells treated with resveratrol at 24h and 48h versus untreated cells (Tables 1, and 2) were performed using the DEVA 1.2.1 software [2]. At 24h treatment, 338 out of 2035 hypermethylated genes correspond to cancer genes; and 92 out of 1738 hypomethylated genes correspond to cancer genes. At 48h treatment, 137 out of 1869 hypermethylated genes were cancer genes; and 288 out of 1661 hypomethylated genes are cancer genes (Tables 3 and 4). In addition, differentially methylated probes and differentially expressed genes were identified between the MDA-MB-231 cells treated with resveratrol at 24h and 48h and the MDA-MB-231 untreated. The integrative analysis of DNA methylation and gene expression at different times of resveratrol exposure showed that changes in DNA methylation were associated to corresponding changes in mRNA expression in a set of cancer-related genes (Tables 5 and 6).
Experimental design, materials and methods
Acknowledgements This work was supported by CONACyT SALUD (233370 and 222335). Rubiceli Medina Aguilar was supported by a CONACYT doctoral fellowship (308746).
Data